siRNA / miRNA gene silencing Mouse MLO‐Y4

Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Mouse - IL-1 beta

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] to perform Immunohistochemistry Human - Hes1

Get tips on using Monoclonal Anti-TBP antibody produced in mouse to perform Western blotting TBP

Get tips on using Monoclonal Anti-MUC5AC antibody produced in mouse to perform Western blotting MUC5AC

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

Get tips on using Purified anti-mouse/rat/human FOXP3 Antibody to perform Western blotting FOXP3