siRNA / miRNA gene silencing Mouse B16-BL6

Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Mouse - IL-1 beta

Get tips on using HES1 Mouse Monoclonal Antibody [Clone ID: OTI1B5] to perform Immunohistochemistry Human - Hes1

Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Rat - OPN

Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2

Get tips on using APC/Cyanine7 anti-mouse I-A/I-E Antibody to perform Flow cytometry Anti-bodies Mouse - MHCII

Get tips on using Brilliant Violet 650™ anti-mouse IFN-γ Antibody to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using Fibroblast Growth Factor 21 Mouse/Rat ELISA to perform ELISA Rat - FGF-21