siRNA / miRNA gene silencing Rat Glial cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat cardiomyocytes

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat MSC

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dorsal root ganglion neurons

Get tips on using AllPrep DNA/RNA Mini Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using Absolutely Total RNA Purification Kits to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts

Get tips on using Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.