Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - RAW264.7
Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - RAW264.7
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HT22
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - NIH3T3
Get tips on using HSP70 ELISA Kit (High-Sensitivity) to perform ELISA Mouse - HSP70
Get tips on using Human BDNF ELISA Kit (ab212166) to perform ELISA Mouse - BDNF
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - RAW 264.7
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay mouse - 3T3-L1
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
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