Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line
Get tips on using Renilla luciferase vector, pGL4.74 to perform Reporter gene assay luciferase - primary human endometrial stromal cells
Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.
Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Human vWF-A2 DuoSet ELISA to perform ELISA Human - VWF-A2
Get tips on using Human PDGF-BB ELISA Kit to perform ELISA Human - PDGF-BB
Get tips on using Human PDGF-BB DuoSet ELISA to perform ELISA Human - PDGF-BB
Get tips on using KIM-1 (human) ELISA kit to perform ELISA Human - KIM-1
Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I
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