RNA sequencing Rat

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Cardiomyocyte (H9C2) HIF-1α Lipid

Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

Get tips on using Silencer® Select_Mapk14/p38 siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Mapk14/p38

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Rat oligodendrocyte precursors (OPCs)

Get tips on using High Pure PCR Template Preparation Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat astrocytes

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 to perform ChIP Rat - Mesenteric arteries

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.