siRNA / miRNA gene silencing Rat INS-1

Get tips on using p-c-Jun Antibody (KM-1): sc-822 to perform Western blotting C-Jun

Get tips on using Human IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Human - IGF-I

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using CD279 (PD-1) Monoclonal Antibody (RMP1-30), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Get tips on using Laminin β-2 Antibody (H-1): sc-133241 to perform Western blotting Laminin subunit Beta-2

Get tips on using AM5770: pSilencer™ 3.1-H1 neo to perform shRNA gene silencing Mouse - CT26 OPN

Get tips on using Laminin beta-2/gamma-1 Monoclonal Antibody (A5) to perform Western blotting Laminin subunit Beta-2

Get tips on using Quick Start™ Bradford Protein Assay Kit 1 to perform Protein quantification Mammalian cells - BV-2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase