Cell line authentication

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using SQSTM1 Antibody (D-3) to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC

Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mast cell

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells

Get tips on using Cytotoxicity Detection KitPLUS (LDH) to perform Cell cytotoxicity / Proliferation assay cell type - HT-22

Get tips on using LDH Cytotoxicity Detection Kit to perform Cell cytotoxicity / Proliferation assay cell type - 3T3-L1

Get tips on using 48-Well Micro Chemotaxis Chamber to perform Cell migration / Invasion cell type - HT-1080