CRISPR Hamster Deletion CHO-K1

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using ResistancePlus® MG FleXible to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hDPSCs differentiation into chondrogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hTSPCs differentiation into Chondrogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hMSCs differentiation into Chondrogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat articular chondrocytes

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,