siRNA / miRNA gene silencing Human PC-3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media PDX mammospheres

Get tips on using SurePrint G3 Human CGH Microarray Kit, 4x180K to perform Microarray Comperative genomic hybridization - Human MDA-MB-453

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Human VEGF ELISA Kit (ab100662) to perform ELISA Human - VEGF

Get tips on using Human VEGF 165b DuoSet ELISA to perform ELISA Human - VEGF

Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1

Get tips on using Human RBP4 Quantikine ELISA Kit to perform ELISA Human - RBP4

Get tips on using Human Prolactin/PRL ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin ELISA Kit (ab100618) to perform ELISA Human - OPN