Microarray Gene expression arrays Human whole blood cells

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Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP

Products Luca Jovine, Karolinska Institutet, Department of Biosciences an pIEX-5-6His-mMBP-UMODpXR

Get tips on using p1.2-Hygro-FSH-B-chain to perform Protein Expression Eukaryotic cells - CHO FSH

Products Ivan I. Vorobiev, Laboratory of Mammalian Cell Bioengineering, I p1.2-Hygro-FSH-B-chain

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Products STEMCELL technologies MammoCult™ Human Medium Kit

Get tips on using STEAP2 metalloreductase to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) STEAP2

Products Thermo Fisher Scientific STEAP2 metalloreductase

RNA siRNA / miRNA gene silencing Rat Astrocytes HMG-1

RNA siRNA / miRNA gene silencing Rat RMC-1 Cx43

RNA siRNA / miRNA gene silencing Rat RGC-5 IGF1R

RNA siRNA / miRNA gene silencing Rat H19-7 EpoR

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Hep3B SFRP3

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