Flow cytometry Anti-bodies Mouse

Get tips on using Endothelin-1 Quantikine ELISA Kit to perform ELISA Mouse - Endothelin 1

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Mouse - Endothelin 1

Get tips on using BMP-2 Quantikine ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Mouse - Chondrocytes

Get tips on using truChIP Chromatin Shearing Kit with Formaldehyde to perform ChIP Mouse - Brain

Get tips on using REDExtract-N-Amp™ PCR ReadyMix™ to perform PCR Mouse

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence) to perform ROS assay cell type - mouse cardiomyocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2