siRNA / miRNA gene silencing Human SUIT-2

Get tips on using FITC Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using PE Mouse Anti-Human CD90 to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using APC anti-human CD24 Antibody to perform Flow cytometry Anti-bodies Human - CD24

Get tips on using BV605 Mouse Anti-Human CD15 to perform Flow cytometry Anti-bodies Human - CD15

Get tips on using PE Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

Get tips on using CRP (Human) ELISA Kit (KA0238) to perform ELISA Human - C-Reactive Protein/CRP

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using PE Mouse Anti-Human CD140a to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2