siRNA / miRNA gene silencing Rat Brain endothelial cells

Get tips on using Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - Hep3B

Get tips on using Dual-Light™ Luciferase & β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - U2OS

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat dermal fibroblasts (rDF)

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Rat mesenteric smooth muscle cells

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat astrocytes

Get tips on using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - CHO-K1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat osteoblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat astrocytes

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.