Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rat prostate tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Lamb muscle tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human placental tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse skeletal muscle
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse prostate tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse liver tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse lung tissue
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - ME epithelial tissue
Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
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