Immunohistochemistry Collagen Type VII

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Collagen I antibody to perform Western blotting Type I collagen

Get tips on using Anti-Collagen X antibody (ab58632) to perform Immunohistochemistry Mouse - Col X

Get tips on using Anti-Collagen II antibody (ab34712) to perform Immunohistochemistry Mouse - Col II

Get tips on using Anti-Collagen III antibody (ab7778) to perform Western blotting Type III collagen

Get tips on using Anti-Collagen I antibody [COL-1] (ab90395) to perform Western blotting Type I collagen

Get tips on using 24-Well Haptotaxis Assays, Collagen I to perform Cell migration / Invasion cell type - HEK293T

Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1

Get tips on using 96-Well Cell Invasion Assay, Collagen I to perform Cell migration / Invasion cell type - MCF-10A