Cell Isolation

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Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Cellular assays Wound healing assay cell type human gHMVEC (glioma human microvascular endothelial cells)

Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - RAW 264.7

Products Sigma-Aldrich Corning® Transwell® polycarbonate membrane cell culture inserts

Get tips on using Oris™ Universal Cell Migration Assembly Kit, 96 wells to perform Cell migration / Invasion cell type - MCF-10A

Products Platypus Technologies Oris™ Universal Cell Migration Assembly Kit, 96 wells

Get tips on using Cultrex 3D Culture BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - BxPC-3

Products Amsbio Cultrex 3D Culture BME Cell Invasion Assay, 96 well

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 5 µm to perform Cell migration / Invasion cell type - SaOS-2

Products Cell Biolabs CytoSelect™ 24-Well Cell Migration Assay, 5 µm

Get tips on using Corning® Transwell® polycarbonate membrane cell culture inserts to perform Cell migration / Invasion cell type - MHCC-97H

Products Sigma-Aldrich Corning® Transwell® polycarbonate membrane cell culture inserts

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - A549

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils

Products Cell Biolabs CytoSelect™ 24-Well Cell Migration Assay, 3 µm

Get tips on using MTT Cell Proliferation Assay (ATCC® 30-1010K™) to perform Cell cytotoxicity / Proliferation assay cell type - MCF-7

Products LGC Standards MTT Cell Proliferation Assay (ATCC® 30-1010K™)

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Stem cell culture media Mice BMSC

Products Lonza EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit

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