siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat astrocytes

Products Qiagen RNeasy Mini Kit

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary rabbit skeletal muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Rat cell lines

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat epidermal keratinocytes

Products Qiagen RNeasy Mini Kit

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat dermal fibroblasts

Products Qiagen RNeasy Mini Kit

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary rat dermal fibroblasts

Products Macherey Nagel NucleoSpin® RNA

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Products Promega FuGENE® 6 Transfection Reagent

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - Retinal ganglion cells (RGCs)

Products Illumina TruSeq RNA Library Prep Kit v2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Deletion K562 c-Myb gene

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