Anti-bodies

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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type RPE-J cells

Get tips on using RNeasy Protect Animal Blood Kit (50) to perform RNA stabilization Blood

Products Qiagen RNeasy Protect Animal Blood Kit (50)
hCas9 Product

Get tips on using hCas9 to perform CRISPR Rat - Activation CD2

Products Addgene hCas9
MLM3636 Product

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA

Products Addgene MLM3636

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF

Products GE Healthcare Life Sciences HiTrap Q FF anion exchange chromatography column

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Products Addgene Cas9m4-VP64
phREX1-Luc Product

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Products Addgene phREX1-Luc

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Clostridium sporogenes

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Candida albicans

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay bacteria - Clostridium sporogenes

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

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