DNA isolation / purification Tissue

Get tips on using EpiTect Fast DNA Bisulfite Kit (200) to perform Bisulfite DNA Modification Fluids

Get tips on using QIAxcel DNA High Resolution Kit (1200) to perform Analysis of DNA fragments

Get tips on using QIAxcel DNA Fast Analysis Kit (3000) to perform Analysis of DNA fragments

Get tips on using BD Cycletest™ Plus DNA Kit to perform DNA quantification Human - HeLa

Get tips on using EpiTect Fast DNA Bisulfite Kit (200) to perform Bisulfite DNA Modification FFPE samples

Get tips on using EpiTect PCR Control DNA Set (100) to perform PCR ORNi-PCR - Plasmid DNA

Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA quantification Human - SH-SY5Y

Get tips on using AmpliTaq Gold™ 360 DNA Polymerase to perform PCR Multiplex PCR - Bacterial DNA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.