PCR ORNi-PCR

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A431

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A253

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

Generally it has been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Use of enzymes to disrupt cell wall can alter gene expression profiles. Therefore, physical disruption can result in high quality RNA for all downstream processing. Use of DNAse and proteinase K will remove traces of DNA contamination and proteins respectively.