rna-isolation-purification-tissue-mouse-small-intestine

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized EL4

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Cells - immortalized B16

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized U373

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized PNT1A

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized MG63

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized Ku812

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized CHO

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.