siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

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IncuCyte® Cell Migration Kit Product

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human HUVEC

Products Essen Bioscience IncuCyte® Cell Migration Kit

BrdU Cell Proliferation Assay Kit Product

Get tips on using BrdU Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Products Cell Signaling Technology BrdU Cell Proliferation Assay Kit

ChIP Human - MDA-MB-231 Experiment

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Human MDA-MB-231

ChIP Human - MIA PaCa-2 Experiment

Proteins ChIP Human MIA PaCa-2

Muse® Cell Cycle Assay Kit Product

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HT-29