Protein Expression Eukaryotic cells T. reesei

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Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Products Biotium Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

Get tips on using GeneChip Rhesus Macaque Genome Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Products Thermo Fisher Scientific GeneChip Rhesus Macaque Genome Array

Get tips on using Qproteome Cell Compartment Kit to perform Protein enrichment Total nuclear proteins

Products Qiagen Qproteome Cell Compartment Kit

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines HEK 293T

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines SH-SY5Y

RNA siRNA / miRNA gene silencing Rat Glial cells GLT-1

RNA siRNA / miRNA gene silencing Rat Neuronal cells MCP-1

Cell culture media 3D Cell Culture Media PLC/PRF/5 cells-spheres

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type human umbelical vein endothelial cells (HUVEC)

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