TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using GeneJET Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp
Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rat skin tissue
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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein smooth muscle cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human umbilical artery smooth muscle cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human pulmonary artery smooth muscle cells
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