rna-isolation-purification-tissue-mouse-small-intestine

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized NTERA-2

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized NK-92MI

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized KBM-7

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized K-562

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized A2780-CIS

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - immortalized MA-104

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Cells - immortalized MA-104

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.