crispr-mouse-deletion-raw-264-7-dcstamp

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Atf4

Get tips on using ON-TARGETplus Rat Prkaa2 (78975) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - UMR‐106 Ampkα2/Prkaa2

Get tips on using mTOR (7C10) Rabbit mAb #2983 to perform Western blotting mTOR

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Color-coded Prestained Protein Marker, Broad Range (10-250 kDa) #74124 to perform Protein Ladder Prestained

Get tips on using IKKγ Antibody #2685 to perform Western blotting IKKgamma

Get tips on using GATA-3 Antibody (HG3-31): sc-268 to perform ChIP Anti-bodies GATA3

Get tips on using Human Genome CGH Microarray 244A Supplemental to perform Microarray Comperative genomic hybridization - Human STUMP