Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using Human Angiopoietin-like 3 DuoSet ELISA to perform ELISA Human - Angiopoietin-Like 3 (AngptL3)

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human BxPC-3

Get tips on using Human Chitinase 3-like 1 DuoSet ELISA to perform ELISA Human - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using BrdU Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - PANC-, BxPC-3 human pancreas