rna-isolation-purification-cells-immortalized-mda-mb-231

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Rat Adrenal glands

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Mouse Mammary glands

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using RNAprep pure Kit (For Plant) to perform RNA isolation / purification Yeast - Neurospora crassa

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Pichia pastoris

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.