rna-isolation-purification-tissue-human-duodenum

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat aortic smooth muscle cells

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using miRNeasy Micro Kit to perform RNA isolation / purification Cells - primary rat cortical neurons

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Candida albicans

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Cryptococcus neoformans

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Pichia pastoris

Get tips on using MasterPureTM-Yeast DNA Purification kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.