Site Directed Mutagenesis (SDM) Human Point mutation PC-3

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized BxPC-3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized SKOV-3

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Caov-3

Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - CaOV-3

Get tips on using Annexin V-FITC Kit to perform Apoptosis assay cell type - OVCAR-3

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.