PCR Quantitative real-time PCR

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Mono-Mac-6

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - Mouse Blood / serum / plasma / buffy coat

Get tips on using MEDOX-Easy Total RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Mouth