DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - RPMI-8226

Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - LP-1

Get tips on using EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) to perform Cell migration / Invasion cell type - LP-1

Get tips on using Propidium iodide solution to perform DNA quantification Human - MDA-MB-231

Get tips on using IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - THP 1

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - BMDM

Get tips on using Human ShhN ELISA to perform ELISA Human - ShhN

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.