Protein expression and purification Bacteria Escherichia coli

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation E. coli DH5α

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation E. coli

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - FaDu

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HaCaT

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.