DNA transfection Mammalian cells - Immortalized cell lines HeLa

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Start discussion

No discussions found

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Found 6 matching solutions for this experiment

Viromer® RED

Lipocalyx GmbH

Upstream tips
For adherent cells, you can target up to 80% confluency at the time of analysis.
Protocol tips
Always add Buffer RED to Viromer® RED, not vice versa!
Upstream tips
Do not include serum and antibiotics during the formation of the ICAFectin 442/siRNA complexes.
Upstream tips
Do not aliquot X-tremeGENE HP DNA Transfection Reagent; store
in the original glass vials.
Lipofectamine® LTX Reagent

Thermo Fisher Scientific

Protocol tips
Do not add antibiotics to the Transfection Medium
Protocol tips
Do not add antibiotics to the Transfection Medium
JetPrime

Polyplus transfections

Protocol tips
Do not add antibiotics to the Transfection Medium
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!
Become shareholder Discussions About us Contact Privacy Terms