Select a Cell type


Cell cycle assay mouse

- Found 8099 results

Cellular assays Cell Isolation CD8 + T cell

Cellular assays Cell Isolation Plasmacytoid Dendritic Cell

Cellular assays Cell Isolation Human NK cell

Cellular assays Cell Isolation CD3+ T Cell

Cellular assays Cell Isolation Naive B cell

Cellular assays Cell Isolation HLA B Cell

Cellular assays Cell Isolation HLA T Cell

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been greatly used for studies on embryonic development and cell differentiation.iPSCs provide a stable source for either self-renewal or differentiation into suitable cells when cultured in a particular environment. Pluripotent cell culture was originally started by deriving cells from inner cell mass (ICM) from pre-implanted blastocysts, these were called embryonic stem cells. These cells after isolation can be grown on traditional extracellular matrices (like mouse embryonic fibroblasts, MEFs) or feeder-free culture systems. DMEM/F12 has been the most commonly used basal media in the culture of pluripotent cells. These cells are cultured at normal atmospheric oxygen levels, 21%, however, some studies have proposed that 4% oxygen tension may be better for hESC growth. Higher D-glucose concentration (4.2g/l) and osmolarity (320mOsm) that mimics the natural environment of embryonic tissue are optimal for the growth of hESCs. Supplements like N2 and/or B-27, in the presence of growth factors like bFGF, have been shown to increase pluripotency of these cells. bFGF, FGF2 and other ligands of receptor tyrosine kinases like IGF are also required or maintain self-renewal ability of these cells. TGF𝛃1, by its activation of SMAD2/3 signalling, also represses differentiation of iPSCs. Other compounds like ROCK inhibitors reduce blebbing and apoptosis in these cells to maintain their clonogenicity. However, an inhibitor for LIF (leukaemia inhibitory factor, which is one of the pluripotent genes) has an opposing effect. Therefore, it is important to understand the culture conditions and media composition that affect downstream signalling in hESCs or iPSCs that may lead to their differentiation.

Cell culture media Stem cell culture media Human bone mesenchymal stem cell (BMSC)

Get tips on using SQSTM1 Antibody (D-3) to perform Cell cytotoxicity / Proliferation assay cell type - K562

Products Santa Cruz Biotechnology SQSTM1 Antibody (D-3)

Get tips on using Thiazolyl Blue Tetrazolium Bromide to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC

Products Sigma-Aldrich Thiazolyl Blue Tetrazolium Bromide

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms