rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using RNAprep pure Kit (For Plant) to perform RNA isolation / purification Yeast - Neurospora crassa

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Primary cells Lymphocytes

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Primary cells Cyst-derived kidney epithelial cells

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Cerebral hemispheres

Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Human Teeth / Jaw