siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using Human MMP-1 Antibody to perform Western blotting MMP1

Get tips on using Adiponectin Human ELISA, High Sensitivity (Sandwich) to perform ELISA Human - Adiponectin

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Human endometrial stromal cells Biotin

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO

Get tips on using Human MMP9 ELISA Kit (lyophilized) (ab100610) to perform ELISA Human - MMP9

Get tips on using Human Decorin ELISA Kit (DCN) (ab99998) to perform ELISA Human - Decorin

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.