PCR Preamplification of cDNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using CpGenome Universal Methylated DNA to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using TopTaq Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using FastLane Cell Multiplex Kit (200) to perform PCR Multiplex PCR - Mammalian DNA

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using QuantiNova IC Probe Assay Red 650 (500) to perform PCR Multiplex PCR - Viral

Get tips on using M-MLV Reverse Transcriptase (200 U/µL) to perform cDNA synthesis Cell lines

Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Bacteria

Get tips on using QuantiNova Pathogen +IC Kit (500) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Bacterial DNA