DNA methylation profiling Gene specific profiling HEK 293T

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Bacteria - Gram positive Weissella confusa

Get tips on using DNeasy PowerLyzer Microbial Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Rhodopseudomonas

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - DAOY GLI1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using Dihydroethidium to perform DNA quantification Human - SH-SY5Y

Get tips on using QIAshredder (250) to perform DNA fragmentation Cell lines

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1

Get tips on using QIAamp MinElute Media Kit to perform DNA isolation / purification Tissue - genital / cervical samples