rna-isolation-purification-cells-immortalized-saos-2

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using QIAamp UltraSens Virus Kit (250) to perform RNA isolation / purification Supernatant from cell cultures

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - human kidney tissue

Get tips on using RNAprep pure Kit (For Plant) to perform RNA isolation / purification Yeast - Neurospora crassa

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Primary cells Lymphocytes

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli