Site Directed Mutagenesis (SDM) Human Point mutation BxPC-3

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3

Get tips on using ANGPTL3 (mouse/rat) Dual ELISA Kit to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - OVCAR-3

Get tips on using single stranded-DNA Apoptosis ELISA kit to perform Apoptosis assay cell type - OVCAR-3

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis SK-BR-3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.