rna-isolation-purification-cells-immortalized-ntera-2

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Yeast - Pichia pastoris

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)