Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

Get tips on using RNeasy PowerMicrobiome Kit (50) to perform RNA isolation / purification Viral - SARS-CoV-2

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized NTERA-2

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Capan-2

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - BV-2

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.