RNA isolation / purification Tissue Human

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - A413

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - H1819

Get tips on using Ion Total RNA-Seq Kit v2 to perform RNA sequencing Human - Huh7

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - Huh7

Get tips on using Ovation® RNA-Seq System V2 to perform RNA sequencing Human - H7

Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HEK293T

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HeLa