Site Directed Mutagenesis (SDM) Human Point mutation Caco-2

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized U-2 OS

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - Sao2-2, MG-62

Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - Saos-2

Get tips on using IEF Marker 3-10, Liquid Mix to perform Protein Ladder IEF and 2-D Standards

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay MIA PaCa-2

Get tips on using Monoclonal Anti-Laminin antibody produced in mouse to perform Western blotting Laminin subunit Beta-2

Get tips on using pSpCas9(BB)-2A-Puro (PX459) V2.0 to perform CRISPR Mouse - Deletion 3T3-L1 fmnl 2/3

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - Sao2-2, MG-62