The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - C2C12
Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Mouse - C2C12
Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - C2C12
Get tips on using HE4 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Repression HE4
Get tips on using dCK CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Human - Repression DCK
Get tips on using MYH9 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Rat - Deletion PC12 myosin IIA (Myh9)
Get tips on using Wwtr1 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Taz
Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI
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