Site Directed Mutagenesis (SDM) Human Point mutation SH-SY5Y

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - DAOY GLI1

Get tips on using Bim siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 Bim

Get tips on using Hs_PLK1_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - A172 PLK1

Get tips on using Hs_HES6_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - A172 HES6

Get tips on using Hs_HES6_1 FlexiTube siRNA. to perform siRNA / miRNA gene silencing Human - A172 HES6

Get tips on using Hs_HDAC5_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - U937 HDAC5

Get tips on using CD151 siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 CD151

Get tips on using FAK siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 FAK

Get tips on using Keratinocyte SFM (1X) to perform 3D Cell Culture Media Human prostate organoid

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.