DNA transfection Mammalian cells Immortalized cell lines

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using EpiTect Fast DNA Bisulfite Kit (200) to perform Bisulfite DNA Modification FFPE samples

Get tips on using EpiTect PCR Control DNA Set (100) to perform PCR ORNi-PCR - Plasmid DNA

Get tips on using RNeasy PowerSoil DNA Elution Kit (25) to perform DNA isolation / purification Soil samples

Get tips on using cobas® DNA Sample Preparation Kit to perform DNA isolation / purification Tissue - lung

Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA quantification Human - SH-SY5Y

Get tips on using AmpliTaq Gold™ 360 DNA Polymerase to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using FragEL™ DNA Fragmentation Detection Kit, Colorimetric - TdT Enzyme to perform TUNEL assay cell type - 3T3 L1 mouse adipose tissue

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.