siRNA / RNAi /miRNA transfection Human Cells HNSCC

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using Human Von Willebrand Factor ELISA Kit (VWF) (ab108918) to perform ELISA Human - VWF-A2

Get tips on using Human Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Human - NGAL/LCN2

Get tips on using Human KIM1 / TIM-1 PicoKine™ ELISA Kit to perform ELISA Human - KIM-1

Get tips on using Human TIM-1/KIM-1/HAVCR DuoSet ELISA to perform ELISA Human - KIM-1

Get tips on using Human IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Human - IGF-I

Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Human - HO-1

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.